Conserved proteins in spermatogenesis: novel markers in disturbed
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Conserved proteins in spermatogenesis: novel markers in disturbed spermatogenesis Dr. Christina Rathke www.bio-rad.com Spermiogenesis includes a series of morphological transformations The histone-to-protamine transition in spermatids histones transi-on proteins protamines H4 hyperacetylation many DNA breaks The histone-to-protamine transition in spermatids histones transi-on proteins protamines H4 hyperacetylation many DNA breaks what else? H3K79 methylation precedes the histone-to-protamine transition anti-H3K79me anti-histones Protamine-mCherry DNA DOT1L expression accompanies chromatin reorganization DNA anti-DOT1L Protamine -mCherry DOT1L proteins in mammals Is DOT1L expressed during spermatogenesis? C57BL/6N DOT1L transcripts accumulate in post-meiotic stages C57BL/6N H3K79 methylation temporally overlaps with histone acetylation anti-DOT1L C57BL/6N H3K79 methylation temporally overlaps with histone acetylation anti-DOT1L H3K79 methylation directly precedes the deposition of transition protein 1 Uropathogenic E. coli (UPEC) infections: severely impaired spermatogenesis e.g. hypo-spermatogenesis, germ cell loss, reduced sperm number but: what happens at the level of chromatin reorganization? UPEC infections lead to: • aberrant spermatid chromatin • premature H3K79me and H4ac • coexistence of H3K79me/H4ac with TNP1 7 days post-UPEC-infection Dottermusch-Heidel C, Gärtner SMK, Tegeder I, Rathke C, Barckmann B, Bartkuhn M, Bhushan S, Steger K, Meinhardt A, Renkawitz-Pohl R. (2014) H3K79 methylation: a new conserved mark that accompanies H4 acetylation prior to histone-to-protamine transition in Drosophila and rat. Biology Open 3: 444-452 Dottermusch-Heidel C, Klaus ES, Gonzalez NH, Bhushan S, Meinhardt A, Bergmann M, Renkawitz-Pohl R, Rathke C, Steger K. (2014) H3K79 methylation directly precedes the histoneto-protamine transition in mammalian spermatids and is sensitive to bacterial infections. Andrology. 2014 Jul 30. doi: 10.1111/j.2047-2927.2014.00248.x. Review: Rathke C, Baarends WM, Awe S, Renkawitz-Pohl R (2014) Chromatin dynamics during spermiogenesis. Biochim Biophys Acta 1839: 155-168 Renkawitz-Pohl R Dottermusch-Heidel C Barckmann B Gärtner SMK Tegeder I Rathke C Gonzalez NH Klaus ES Bartkuhn M Bergmann M Steger K Meinhardt A Bhushan S KFO181 LOEWE-Schwerpunkt "MIBIE - Männliche Infertilität bei Infektion & Entzündung"
