Veröffentlichungen 2009 Abstracts FB Biotechnologie, Institut für Nutztiergenetik Friedrich-Loeffler-Institut Bermejo-Alvarez P, Rizos D, Rath D, Lonergan P, Gutierrez-Adan A: Microarray analysis reveals that one third of the genes actively expressed are differentially expressed between male and female bovine blastocysts. Biol Reprod 81 (Suppl 1), 40 Buranaamnuay K, Großfeld R, Struckmann C, Rath D: Use of glycerol and amides, along with antioxidants fails to improve quality of frozen-thawed boar spermatozoa. Reprod Dom Anim 44 (Suppl 3), 97 Cunha A, Lemme E, Lucas-Hahn A, Queisser AL, Hassel P, Petersen B, Niemann H: Towards the generation of human TFPI transgenic pigs with improved features for xenotransplantation. Xenotransplantation 16, 434 Drallmeyer S, Müller K, Hadeler KG, Korsawe K, Niemann H, Wrenzycki C: DNA methyltransferase (DNMT)1 and DNMT3a protein expression in pre-implantation bovine embryos derived in vivo and in vitro. Reprod Fertil Dev 21, 144 Garrels W, Kues W, Baulain U, Niemann H: Experimental modulation of telomerase activity in bovine embryos. Reprod Dom Anim 44 (Suppl. 3), 85 Garrels W, Kues W, Baulain U, Niemann H: Experimental modulation of telomerase activity in bovine embryos. Proc 5th NRW Stem Cell Meeting, 49 Haridoss S, Niemann H, Kues W, Moritz T, Cantz T, Martin U, Schambach A, Sgodda M, Jagielska J, Pfaff N, Luettge D, Nowak-Imialek M: Generation and characterisation of porcine induced pluripotent stem cells. Hum Gene Therapy 20, 1523 Heinzmann J, Hansmann T, Wrenzycki C, Zechner U, Haaf T, Niemann H: Epigenetische Analyse geprägter Gene in bovinen Eizellen unterschiedlichen Ursprungs. Proc. Vortragstagung DGfZ und GfT 2009 Gießen, 48-49 Hinkel R, Petersen B, Horstkotte J, Wuchrer A, Schmöckel M, Niemann H, Kupatt C: hHO-1 overexpression in transgenic pigs reduces ischemia/reperfusion injury after acute myocardial infarction. Xenotransplantation 16, 359 Honnens Ä, Klein C, Hadeler KG, Niemann H, Bollwein H, Wrenzycki C: Doppler sonography of the ovarian arteries in superovulated cattle. Reprod Fertil Dev 21, 244-245 Honnens Ä, Klein C, Niemann H, Bollwein H, Wrenzycki C: Effect of dominant follicle ablation on the relationship between follicular blood flow and embryo yield in superovulated cattle. Reprod Dom Anim 44 (Suppl 1), 16 Iqbal K, Kues WA, Niemann H: Plasmid-mediated experimental telomere extension in bovine embryos by ectopic expression of human TERT. Reprod Fertil Dev 21, 235-236 Iqbal K, Kues WA, Niemann H: Plasmid-mediated experimental telomere extension in bovine embryos by ectopic expression of human TERT (hTERT). Reprod Dom Anim 44 (Suppl 1), 17 Iqbal K, Barg-Kues B, Niemann H, Kues WA: Mammalian embryos respond differentially to 5-Cytosine Methylation at CpG or GpC. Proc. Stem Cell Network North Rhine-Westphalia, 36 Iqbal K, Barg-Kues B, Niemann H, Kues WA: Mammalian embryos discriminate between 5-cytosine methylation at CpG and GpC dinucleotides. Proc. EMBO Meeting on Chromatin and Epigenetics, May 2009, Heidelberg, 142 Jobst D, Petersen B, Kues WA, Kurth R, Niemann H, Denner J: Long-term characterisation of transgenic pigs expressing PERV-specific shRNA with knock-down of porcine endogenous retroviruses (PERVs). Xenotransplantation 16, 384 -1- Veröffentlichungen 2009 Abstracts FB Biotechnologie, Institut für Nutztiergenetik Friedrich-Loeffler-Institut Kues WA, Iqbal K, Barg-Kues B, Niemann H: Mammalian embryos discriminate between 5-Cytosine methylation at CpG or GpC dinucleotides. Proc. EMBO Conf. Series on Chromatin and Epigenetics, 142 Kues WA, Iqbal K, Barg-Kues B, Niemann H: Reprogramming events in early bovine and murine embryos are mirrored by plasmid-encoded marker constructs. Reprod Fertil Dev 21, 249-250 Kütemeyer K, Lucas-Hahn A, Petersen B, Hassel P, Lemme E, Niemann H, Heisterkamp A: Femtosecond laser based enucleation of porcine oocytes for somatic cell nuclear transfer. Hum Gene Therapy 20, 1515 Lebzien P, Meyer U, Niemann H, Hachenberg S: Strategien zur Minderung von klimarelevanten Emissionen und N-Ausscheidungen aus der Tierproduktion. BMELV/vTIFachtagung „Aktiver Klimaschutz und Anpassung an den Klimawandel – Beiträge der Agrarund Forstwirtschaft“. Abstractband S, 32-34 Matthes K, Menke D, Köhler P, Niemann H, Brugge WR, Hochberger J: Feasibility of Endoscopic Transgastric (ETGN) and Transvaginal (ETVN) Nephrectomy. Gastrointestinal Endoscopy 65, AB290 Müller K, Herrmann D, Drallmeyer S, Hadeler KG, Korsawe K, Niemann H, Wrenzycki C: In vivo cultured bovine embryos have a similar mRNA expression pattern compared to their in vivo generated counterparts. Reprod Dom Anim 44 (Suppl 1), 25 Niemann H: Klonen: Perspektiven für die Nutztierhaltung. Deutsche Bauern Korrespondenz 10, 16-17 Niemann H, Carnwath JW, Herrmann D, Lemme E, Lucas-Hahn A, Wieczorek G, Olek S: DNA methylation patterns discriminate between bovine somatic cell nuclear transfer embryos, embryos produced by in vitro fertilization and embryos developing in vivo. EMBO Conf. Series on Chromatin and Epigenetics, 169 Niemann H, Dänicke S, Conraths FJ, Schrader L: Tierzucht und Tierhaltung: Anpassung an den Klimawandel aus der Sicht der Erzeugung von Lebensmitteln tierischer Herkunft. Proc. „Aktiver Klimaschutz und Anpassung an den Klimawandel“, Beiträge der Agrar- und Forstwirtschaft, 53-55 Niemann H, Petersen B, Lucas-Hahn A, Lemme E, Oropeza M, Queißer AL, Herrmann D, Kues W, Barg-Kues B, Carnwath JW: Production and characterization of multitransgenic pigs for xenotransplantation. Proc. Transgenic animal research conference VII, 4445 Niemann H, Wieczorek G, Olek S, Carnwath JW, Herrmann D, Lemme E, Lucas-Hahn A: DNA methylation patterns discriminate between bovine somatic cell nuclear transfer embryos, embryos produced by in vitro fertilization and embryos developing in vivo. Proc. EMBO Conference Series on Chromatin and Epigenetics, 169 Niemann H: Unravelling the transcriptome of bovine embryos derived in vivo: Setting the standard for in vitro studies. Cost Gemini Conf. Proc., Maternal communication with Gametes and Embryo, 19 Nowak-Imialek M, Kues W, Petersen B, Lucas-Hahn A, Herrmann D, Lemme E, Oropeza M, Carnwath JW, Niemann H: Oct4/EGFP transgenic pigs – large animal model for reprogramming studies. Proc. 7th Annual Meeting ISSCR 2009, Barcelona, Spain, 230 Nowak-Imialek M, Kues W, Petersen B, Lucas-Hahn A, Herrmann D, Lemme E, Oropeza M, Carnwath J, Niemann H: Generation of Oct4 promoter EGFP transgenic pigs by somatic nuclear transfer. Hum Gene Therapy 20, 1525 -2- Veröffentlichungen 2009 Abstracts FB Biotechnologie, Institut für Nutztiergenetik Friedrich-Loeffler-Institut Nowak-Imialek M, Kues W, Petersen B, Lucas-Hahn A, Herrmann D, Lemme E, Oropeza M, Carnwath J, Niemann H: Oct4-EGFP transgene Schweine – ein Großtiermodel für Reprogrammierungsstudien. Proc. Vortragstagung DGfZ und GfT 2009, Gießen, 164-165 Nowak-Imialek M, Kues WA, Petersen B, Lucas-Hahn A, Oropeza M, Carnwath JW, Niemann H: Generation of Oct4-GFP transgenic pig lines. Proc 5th NRW Stem Cell Meeting, 109 Oropeza M, Petersen B, Carnwath JW, Lucas-Hahn A, Lemme E, Hassel P, Herrmann D, Barg-Kues B, Holler S, Baars W, Hinkel R, Schwinzer R, Kupatt C, Niemann H: Production and characterization of hA20-transgenic pigs suitable for xenotranspIantation. Xenotransplantation 16, 375 Oropeza M, Petersen B, Lemme E, Lucas-Hahn A, Queisser AL, Herrmann D, Hassel P, Niemann H: Generation and characterization of human A20 transgenic pigs by somatic cell nuclear transfer. Reprod Fertil Dev 21, 250 Petersen B, Lucas-Hahn A, Lemme E, Queisser AL, Oropeza M, Herrmann D, Kues WA, Barg-Kues B, Baars W, Schwinzer R, Niemann H: Generation and characterization of human hemeoxygenase-1 (hHO-1) transgenic pigs. Xenotransplantation 16, 373 Petersen B, Lucas-Hahn A, Lemme E, Queisser AL, Oropeza M, Herrmann D, Kues W, Barg-Kues B, Niemann H: Generation and characterization of pigs transgenic for human hemeoxygenase-1 (hHO-1). Proc. DAX Meeting Berlin, 6-7 Petrovicova I, Østrup O, Strejcek F, Lucas-Hahn A, Lemme E, Niemann H, Laurincik J, Maddox-Hyttel P: Nuclear and Nucleolar Remodelling during the First Cell Cycle in Bovine Somatic Cell Nuclear Transfer and Tetraploid Embryos. Reprod Dom Anim 44 (Suppl. 3), 120 Pfaff N, Jagielska J, Sgodda M, Lüttge D, Lachmann N, Warlich E, Baum C, Niemann H, Cantz T, Schambach A, Moritz T: Generation of induced pluripotent stem cells (iPS) from murine bone marrow. Hum Gene Therapy 20, 1525-1526 Pfaff N, Jagielska J, Sgodda M, Lüttge D, Lachmann N, Warlich E, Baum C, Niemann H, Cantz T, Schambach A, Moritz T: Generation of induced pluripotent stem cells (iPS) from murine bone marrow. Proc 5th NRW Stem Cell Meeting, 110 Racedo SE, Rawe VY, Niemann H: Dynamics of Golgi apparatus during bovine oocyte in vitro maturation: effects of inhibitors on CDC2A and cytoplasmic-dynein ATPase activity. Reprod Fertil Dev 21, 225 Racedo SE, Rawe VY, Niemann H: Dynamics of Golgi apparatus during bovine oocyte in vitro maturation: effects of inhibitors on CDC2A and cytoplasmic-dynein ATPase activity. Reprod Dom Anim 44 (Suppl 1), 27 Ruiz S, Zaraza J, De Ondiz A, Rath D: Early fertilization events in bovine IVF employing OPU-oocytes and sex-sorted frozen/thawed sperm. Reprod Dom Anim 44 (Suppl 3), 123 Ruiz S, Zaraza J, De Ondiz A, Rath D: Short co-incubation time in bovine IVF with OPU oocytes and sex sorted/unsorted spermatozoa. Proc. 60th Annual EAAP Annual Meeting, Barcelona, Spain, 405 Schwinzer R, Baars W, Lieke T, Zimmermann R, Petersen B, Kues W, Niemann H: Efficiency of genetic modifications to prevent antibody/complement- and cell-mediated lysis of pig cells. Xenotransplantation 16, 363 -3- Veröffentlichungen 2009 Abstracts FB Biotechnologie, Institut für Nutztiergenetik Friedrich-Loeffler-Institut Taylor U, Kues W, Rath D: In situ hybridisation of telomere sequences in condensed and decondensed bovine spermatozoa before and after sex sorting. Reprod Dom Anim 44 (Suppl 3), 128 Taylor U, Petersen S, Klein S, Barcikowski S, Rath D: Konfokale Visualisierung von lasergenerierten Goldnanopartikeln in somatischen Zellen. Proc. Vortragstagung DGfZ und GfT Gießen, 112-113 Taylor U, Zerbe H, Seyfert HM, Rath D, Schuberth HJ: Binding of porcine spermatozoa to uterine epithelial cells modulates the female immune response and might indicate the formation of a pre-oviductal sperm reservoir. Soc Reprod Fertil 66 (Suppl), 83-84 Ulrichs K, Chodnevskaja I, Petersen B, Niemann H, Wolf E, Reichenbach HD, Lindner P, Fuchs A: Quality criteria for porcine islet cells suitable for xenotransplantation. Proc. DAX Meeting 2009, Berlin Velazquez MA, Hadeler KG, Beckers JF, Remy B, Niemann H: Effects of intraovarian application of insulin-like growth factor-1 (IGF-1) on the superovulatory response of diary cattle. Reprod Dom Anim 44 (Suppl 1), 38 Velazquez MA, Niemann H: Apoptosis and development of in vitro-produced bovine embryos exposed to supraphysiological concentrations of insulin-like growth factor-1 (IGF1). Reprod Fertil Dev 21, 165 Zaraza J, Korsawe K, Herrmann D, Niemann H: In vitro embryo production of pre- and post-pubertal superstimulated dairy heifers after intraovarian injection of insulin-like growth factor-1 (IGF-1). Reprod Dom Anim 44 (Suppl 1), 42 -4-