KIM ET AL., BIOTECHNOL. BIOENG., vol. 93, no. 2, 2006, pages
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Title (en) Process for the asymmetric amination of ketones employing omega-transaminases in organic solvents Title (de) Verfahren zur asymmetrischen Aminierung von Ketonen unter Verwendung von omega-Transamminasen in organischen Lösungsmitteln Title (fr) Procédé d'amination asymmétrique de cétones en utilisant des oméga-transaminases dans des solvants organiques Publication EP 2641972 A1 20130925 (DE) Application EP 12160810 A 20120322 Priority EP 12160810 A 20120322 Abstract (en) Preparing amine compounds (I), comprises aminating ketone compounds (II) by enzymatic catalysis using omega -transaminase comprising pyridoxal-5'-phosphate as a cofactor and an amine as an amino-donor, in an organic solvent, in which water concentration is adjusted between 0 and saturation concentration of solvent, before carrying out the amination reaction, such that water concentration is greater than 0 and less than saturation concentration of the solvent. Preparing amine compounds of formula (R-CH(NH 2)-R1) (I), comprises aminating ketone compounds of formula (R-C(=O)-R1) (II) by enzymatic catalysis using omega -transaminase comprising pyridoxal-5'-phosphate as a cofactor and an amine as an amino-donor, in an organic solvent, in which water concentration is adjusted between 0 and saturation concentration of solvent, before carrying out the amination reaction, such that water concentration is greater than 0 and less than saturation concentration of the solvent. R, R1 : monovalent hydrocarbyl. Abstract (de) Die Erfindung betrifft ein Verfahren zur Herstellung eines Amins (3) durch Aminierung eines Ketons (1) mittels enzymatischer Katalyse unter Verwendung einer É-Transaminase (É-TA) als Katalysator, von Pyridoxal-5'-phosphat (PLP) als Cofaktor und eines Amins (2) als Amino-Donor, wobei die nachstehende Reaktion in einem organischen Lösungsmittel durchgeführt wird: worin R und R' einwertige Kohlenwasserstoffreste sind, mit dem Kennzeichen, dass im organischen Lösungsmittel eine zwischen 0 und der Sättigungskonzentration c S des jeweiligen Lösungsmittels liegende Wasserkonzentration c W eingestellt wird, bevor die Aminierungsreaktion durchgeführt wird, so dass gilt: 0 < Cw < c S . Inventor • MUTTI FRANCESCO (AT) • KROUTIL AO UNIV PROF DI WOLFGANG (AT) Applicant UNIV GRAZ (AT) IPC 8 full level (invention and additional information) C12P 13/00 (2006.01) CPC (invention and additional information) C12P 13/001 (2013.01) Citation (applicant) KIM ET AL., BIOTECHNOL. BIOENG., vol. 93, no. 2, 2006, pages 391 - 5 Citation (search report) • [IDA] YUN, H. ET AL.: "Synthesis of Enantiomerically Pure trans-(1R,2R)- and cis-(1S,2R)-1-Amino-2-Indanol by Lipase and omegaTransaminase", BIOTECHNOLOGY AND BIOENGINEERING, vol. 93, no. 2, 5 February 2006 (2006-02-05), pages 391 - 395, XP002685396 • [A] DORDICK, J.S. ET AL.: "Enzymatic catalysis in monophasic organic solvents", ENZYME AND MICROBIAL TECHNOLOGY, vol. 11, no. 4, April 1989 (1989-04-01), pages 194 - 211, XP023791034 Designated contracting state (EPC) AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR Designated extension state (EPC) BA ME EPO simple patent family EP 2641972 A1 20130925; WO 2013139936 A1 20130926 INPADOC legal status 2014-09-24 [18D] DEEMED TO BE WITHDRAWN - Ref Legal Event Code : 18D - Effective date : 20140326 2013-09-25 [AK] DESIGNATED CONTRACTING STATES: - Ref Legal Event Code : AK - Designated State(s) : AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR 2013-09-25 [AX] REQUEST FOR EXTENSION OF THE EUROPEAN PATENT TO - Ref Legal Event Code : AX - Extension state : BA ME
