KIM ET AL., BIOTECHNOL. BIOENG., vol. 93, no. 2, 2006, pages

Title (en)
Process for the asymmetric amination of ketones employing omega-transaminases in organic solvents
Title (de)
Verfahren zur asymmetrischen Aminierung von Ketonen unter Verwendung von omega-Transamminasen in organischen Lösungsmitteln
Title (fr)
Procédé d'amination asymmétrique de cétones en utilisant des oméga-transaminases dans des solvants organiques
Publication
EP 2641972 A1 20130925 (DE)
Application
EP 12160810 A 20120322
Priority
EP 12160810 A 20120322
Abstract (en)
Preparing amine compounds (I), comprises aminating ketone compounds (II) by enzymatic catalysis using omega -transaminase comprising
pyridoxal-5'-phosphate as a cofactor and an amine as an amino-donor, in an organic solvent, in which water concentration is adjusted between 0
and saturation concentration of solvent, before carrying out the amination reaction, such that water concentration is greater than 0 and less than
saturation concentration of the solvent. Preparing amine compounds of formula (R-CH(NH 2)-R1) (I), comprises aminating ketone compounds of
formula (R-C(=O)-R1) (II) by enzymatic catalysis using omega -transaminase comprising pyridoxal-5'-phosphate as a cofactor and an amine as an
amino-donor, in an organic solvent, in which water concentration is adjusted between 0 and saturation concentration of solvent, before carrying out
the amination reaction, such that water concentration is greater than 0 and less than saturation concentration of the solvent. R, R1 : monovalent
hydrocarbyl.
Abstract (de)
Die Erfindung betrifft ein Verfahren zur Herstellung eines Amins (3) durch Aminierung eines Ketons (1) mittels enzymatischer Katalyse unter
Verwendung einer É-Transaminase (É-TA) als Katalysator, von Pyridoxal-5'-phosphat (PLP) als Cofaktor und eines Amins (2) als Amino-Donor,
wobei die nachstehende Reaktion in einem organischen Lösungsmittel durchgeführt wird: worin R und R' einwertige Kohlenwasserstoffreste sind, mit
dem Kennzeichen, dass im organischen Lösungsmittel eine zwischen 0 und der Sättigungskonzentration c S des jeweiligen Lösungsmittels liegende
Wasserkonzentration c W eingestellt wird, bevor die Aminierungsreaktion durchgeführt wird, so dass gilt: 0 < Cw < c S .
Inventor
• MUTTI FRANCESCO (AT)
• KROUTIL AO UNIV PROF DI WOLFGANG (AT)
Applicant
UNIV GRAZ (AT)
IPC 8 full level (invention and additional information)
C12P 13/00 (2006.01)
CPC (invention and additional information)
C12P 13/001 (2013.01)
Citation (applicant)
KIM ET AL., BIOTECHNOL. BIOENG., vol. 93, no. 2, 2006, pages 391 - 5
Citation (search report)
• [IDA] YUN, H. ET AL.: "Synthesis of Enantiomerically Pure trans-(1R,2R)- and cis-(1S,2R)-1-Amino-2-Indanol by Lipase and omegaTransaminase", BIOTECHNOLOGY AND BIOENGINEERING, vol. 93, no. 2, 5 February 2006 (2006-02-05), pages 391 - 395,
XP002685396
• [A] DORDICK, J.S. ET AL.: "Enzymatic catalysis in monophasic organic solvents", ENZYME AND MICROBIAL TECHNOLOGY, vol.
11, no. 4, April 1989 (1989-04-01), pages 194 - 211, XP023791034
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EPO simple patent family
EP 2641972 A1 20130925; WO 2013139936 A1 20130926
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